The 7th Division's Private Hyakunosuke Ogata and former Shinsengumi Vice Commander Toshizou Hijikata find themselves on opposite sides of a gang war in Barato after hearing rumors of the Hidoro gang possessing an escaped prisoner's tattooed skin. With a lead to the hidden Ainu gold close at hand, the two gladly take up arms but a betrayal will force both sides to think twice before carelessly jumping the gun.

Golden Kamuy OVA is an original animation bundled with the 15th volume of the Golden Kamuy manga. The animation takes place during the events of the final episode of season one, but follows Hyakunosuke Ogata and Toshizou Hijikata.