The Imperial Fighting Troupe is called again to defend Tokyo against the demons. However, this time, the Douglas-Stewart Company is selling a superior fighting machine that will revolutionize Japan's defense and make the Flower Division obsolete. However, the girls suspect something may be wrong when members of their team begin to vanish! But what can they do when their division has been placed on indefinite stand-by status?

Sakura Taisen: Katsudou Shashin takes place between the games Sakura Taisen 3 and Sakura Taisen 4.