Yuko Kondou is the daughter of Isami Kondo and field commander of the Mobile Shinsengumi. She has a habit of breaking her sword in battle, and a tendency to rack up debts wherever she goes. Yuko is slightly scatterbrained and has pink hair, and enjoys making fun of Okita's breasts.

Kondo seems to be the fondest of the three girls of Oryou, since she frequently imagines the older woman committing suicide if Ryuunosuke were to return to Shanghai. To prevent this from happening, she comes up with various harebrained ideas to keep the young man in Kyoto by making him fall in love with a local waitress named Sayoko. Most of her plans to make the pair fall in love fail miserably, and soon her plans become less about preventing his departure and more about succeeding.