She supervises the class next to Tsuchida's. Tsuchida fell in love with her at first sight.

Yamamoto-sensei is one of the female teachers at Hanamaru Kindergarten. Even though Tsuchida likes her, she does not understand that men would fall for her. She teaches the students of first-year Peach Class. She appears to be good at sewing, as she has fixed Tsuchida's apron and Koume's stuffed panda-cat doll's ear.